Unbiased homeologous recombination during pneumococcal transformation allows for multiple chromosomal integration events

نویسندگان
چکیده

برای دانلود رایگان متن کامل این مقاله و بیش از 32 میلیون مقاله دیگر ابتدا ثبت نام کنید

اگر عضو سایت هستید لطفا وارد حساب کاربری خود شوید

منابع مشابه

Homeologous plastid DNA transformation in tobacco is mediated by multiple recombination events.

Efficient plastid transformation has been achieved in Nicotiana tabacum using cloned plastid DNA of Solanum nigrum carrying mutations conferring spectinomycin and streptomycin resistance. The use of the incompletely homologous (homeologous) Solanum plastid DNA as donor resulted in a Nicotiana plastid transformation frequency comparable with that of other experiments where completely homologous ...

متن کامل

Mismatch repair during homologous and homeologous recombination.

Homologous recombination (HR) and mismatch repair (MMR) are inextricably linked. HR pairs homologous chromosomes before meiosis I and is ultimately responsible for generating genetic diversity during sexual reproduction. HR is initiated in meiosis by numerous programmed DNA double-strand breaks (DSBs; several hundred in mammals). A characteristic feature of HR is the exchange of DNA strands, wh...

متن کامل

Conversion of deletions during recombination in pneumococcal transformation.

Genetic analysis of 16 deletions obtained in the amiA locus of pneumococcus is described. When present on donor DNA, all deletions increased drastically the frequency of wild-type recombinants in two-point crosses. This effect was maximal for deletions longer than 200 bases. It was reduced for heterologies shorter than 76 bases and did not exist for very short deletions. In three-point crosses ...

متن کامل

Programmed Protection of Foreign DNA from Restriction Allows Pathogenicity Island Exchange during Pneumococcal Transformation

In bacteria, transformation and restriction-modification (R-M) systems play potentially antagonistic roles. While the former, proposed as a form of sexuality, relies on internalized foreign DNA to create genetic diversity, the latter degrade foreign DNA to protect from bacteriophage attack. The human pathogen Streptococcus pneumoniae is transformable and possesses either of two R-M systems, Dpn...

متن کامل

On the Role of Integrity of Dna Particles in Genetic Recombination during Pneumococcal Transformation.

8 The term "freezing of the particles" is used for convenience. As it is uncertain whether or not there is an alteration in the physical state of the particles when frozen at -20'C, the term is therefore descriptive of the state of the suspending medium. 9 Weber, M. M., and G. Rosso, Fed. Proc., 22, 526 (1963). lo Gale, P. H., B. H. Arison, N. R. Trenner, A. C. Page, Jr., K. Folkers, and A. F. ...

متن کامل

ذخیره در منابع من


  با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید

ژورنال

عنوان ژورنال: eLife

سال: 2020

ISSN: 2050-084X

DOI: 10.7554/elife.58771